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paper n a paav hsyn egfp dr bryan roth addgene plasmid  (Addgene inc)


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    Structured Review

    Addgene inc paper n a paav hsyn egfp dr bryan roth addgene plasmid
    Paper N A Paav Hsyn Egfp Dr Bryan Roth Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dr+bryan+roth/pAAV-hSyn-EGFP+(Plasmid+%2350465)/pm39357522-886-163-169
    Average 96 stars, based on 374 article reviews
    paper n a paav hsyn egfp dr bryan roth addgene plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Basal Forebrain Cholinergic Neurons are Part of the Threat Memory Engram
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti ChAT (1:500) Abcam Cat#ab18736 Rabbit anti GFP (1:1000) Invitrogen Cat#A11122 Chicken anti GFP (1:500) Abcam Cat#ab3970 Mouse anti mCherry (1:500) Takara Cat#632543 Rabbit anti DsRed (1:500) Takara Cat#632496 Rabbit anti cFos (1:500) Synaptic Systems Cat#226003 Donkey anti Rabbit IgG, Alexa Fluor 488 (1:1000) ThermoFisher Cat#A32790 Donkey anti Rabbit IgG, Rhodamine Red-X (1:1000) Jackson Immunoresearch RRID: AB_2340613 Donkey anti Goat IgG, Alexa Fluor 594 (1:1000) ThermoFisher Cat#A-11058 Donkey anti Chicken IgG, Cy2 (1:1000) Gift from Dr.Shaoyu Ge, Stony Brook University, NY Neurotrace 435/455 (1:500) ThermoFisher Cat#N21479 Bacterial and Virus Strains CAV2-DIO-hM4Di-mCherry Dr.EJ Kremer, Institut de Génétique Moléculaire de Montpellier, France. .. AAV9-PCamk2aGCaMP6f.WPRE.SV40 U. Penn Vector Core AAV8-DIO-hM4Di-mCherry Addgene Cat#44362 AAV9-DIO-eCFP Vector Labs AAV9-GACh4.3 Vigene Biosciences Inc. Stellar Competent Cells, HST08 strain Takara Cat#636766 AAV9-TRE-DIO-oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript AAV9-TRE-DIO-hM4Di-mCherry This Manuscript Chemicals, Peptides, and Recombinant Proteins T4 DNA Ligase NEB Cat#M0202S BglII NEB Cat#R0144S AscI NEB Cat#R0558S BamHI-HF NEB Cat#R3136S PmlI NEB Cat#R0532S Fast Blue Polysciences Inc. Cat#17740-1 doxycycline hyclate-containing chow Envigo Cat#TD.08541 Mt.Lion Pee Maine outoor solutions LLC Clozapine Sigma Aldrich Cat#C6305-25MG Autofluorescence eliminator reagent Millipore Sigma Cat#2160 Phusion® High-Fidelity DNA Polymerase NEB Cat#M0530S Electronic copy available at: https://ssrn.com/abstract=3848894 Critical Commercial Assays In-Fusion® HD Cloning Plus Takara Cat#638920 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Experimental Models: Organisms/Strains Mouse: Chat-IRES-Cre (B6;129S6Chattm2(cre)Lowl/J) The Jackson Laboratory Jax stock number: 006410 Mouse: TetTag (B6;DBA-Tg(FostTA,Fos-EGFP*)1Mmay) The Jackson Laboratory Jax stock number: 008344 Oligonucleotides Chat FWD: TCTGGCAACTTCGTCGGA, REV: CTCCTGGGCTGTTACGCAC IDT pV2.1 - Gene Block 7xTetO-LoxP-Lox2272tTAH100Y.SV40 IDT pV2.2- Gene Block oChIEF-LoxP-Lox2272 IDT Recombinant DNA pAAV-hSyn-DIO-hM4D(Gi)mCherry Addgene, gift from Dr.Bryan Roth Cat#44362 pV2SGE Gift from Dr.Shaoyu Ge, Stony Brook University NY. pAAV-TRE-DIO--oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript pAAV-TRE-DIO-hM4Di-mCherry This Manuscript Software and Algorithms Prism GraphPad Software Inc. Sigmaplot 12.5 Systat Software Inc. OriginPro 9.1 Origin Lab Corporation Fiji is just imagej Fiji FreezeFrame v3 Actimetrics MATLAB Mathworks Pre-processing Analysis MATLAB Script for FiberPhotometry Doric ACh sensor analysis MATLAB script Crouse, Richard B., et al. "Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency." ..

    Cloning:

    Article Title: Basal Forebrain Cholinergic Neurons are Part of the Threat Memory Engram
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti ChAT (1:500) Abcam Cat#ab18736 Rabbit anti GFP (1:1000) Invitrogen Cat#A11122 Chicken anti GFP (1:500) Abcam Cat#ab3970 Mouse anti mCherry (1:500) Takara Cat#632543 Rabbit anti DsRed (1:500) Takara Cat#632496 Rabbit anti cFos (1:500) Synaptic Systems Cat#226003 Donkey anti Rabbit IgG, Alexa Fluor 488 (1:1000) ThermoFisher Cat#A32790 Donkey anti Rabbit IgG, Rhodamine Red-X (1:1000) Jackson Immunoresearch RRID: AB_2340613 Donkey anti Goat IgG, Alexa Fluor 594 (1:1000) ThermoFisher Cat#A-11058 Donkey anti Chicken IgG, Cy2 (1:1000) Gift from Dr.Shaoyu Ge, Stony Brook University, NY Neurotrace 435/455 (1:500) ThermoFisher Cat#N21479 Bacterial and Virus Strains CAV2-DIO-hM4Di-mCherry Dr.EJ Kremer, Institut de Génétique Moléculaire de Montpellier, France. .. AAV9-PCamk2aGCaMP6f.WPRE.SV40 U. Penn Vector Core AAV8-DIO-hM4Di-mCherry Addgene Cat#44362 AAV9-DIO-eCFP Vector Labs AAV9-GACh4.3 Vigene Biosciences Inc. Stellar Competent Cells, HST08 strain Takara Cat#636766 AAV9-TRE-DIO-oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript AAV9-TRE-DIO-hM4Di-mCherry This Manuscript Chemicals, Peptides, and Recombinant Proteins T4 DNA Ligase NEB Cat#M0202S BglII NEB Cat#R0144S AscI NEB Cat#R0558S BamHI-HF NEB Cat#R3136S PmlI NEB Cat#R0532S Fast Blue Polysciences Inc. Cat#17740-1 doxycycline hyclate-containing chow Envigo Cat#TD.08541 Mt.Lion Pee Maine outoor solutions LLC Clozapine Sigma Aldrich Cat#C6305-25MG Autofluorescence eliminator reagent Millipore Sigma Cat#2160 Phusion® High-Fidelity DNA Polymerase NEB Cat#M0530S Electronic copy available at: https://ssrn.com/abstract=3848894 Critical Commercial Assays In-Fusion® HD Cloning Plus Takara Cat#638920 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Experimental Models: Organisms/Strains Mouse: Chat-IRES-Cre (B6;129S6Chattm2(cre)Lowl/J) The Jackson Laboratory Jax stock number: 006410 Mouse: TetTag (B6;DBA-Tg(FostTA,Fos-EGFP*)1Mmay) The Jackson Laboratory Jax stock number: 008344 Oligonucleotides Chat FWD: TCTGGCAACTTCGTCGGA, REV: CTCCTGGGCTGTTACGCAC IDT pV2.1 - Gene Block 7xTetO-LoxP-Lox2272tTAH100Y.SV40 IDT pV2.2- Gene Block oChIEF-LoxP-Lox2272 IDT Recombinant DNA pAAV-hSyn-DIO-hM4D(Gi)mCherry Addgene, gift from Dr.Bryan Roth Cat#44362 pV2SGE Gift from Dr.Shaoyu Ge, Stony Brook University NY. pAAV-TRE-DIO--oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript pAAV-TRE-DIO-hM4Di-mCherry This Manuscript Software and Algorithms Prism GraphPad Software Inc. Sigmaplot 12.5 Systat Software Inc. OriginPro 9.1 Origin Lab Corporation Fiji is just imagej Fiji FreezeFrame v3 Actimetrics MATLAB Mathworks Pre-processing Analysis MATLAB Script for FiberPhotometry Doric ACh sensor analysis MATLAB script Crouse, Richard B., et al. "Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency." ..

    Reverse Transcription:

    Article Title: Basal Forebrain Cholinergic Neurons are Part of the Threat Memory Engram
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti ChAT (1:500) Abcam Cat#ab18736 Rabbit anti GFP (1:1000) Invitrogen Cat#A11122 Chicken anti GFP (1:500) Abcam Cat#ab3970 Mouse anti mCherry (1:500) Takara Cat#632543 Rabbit anti DsRed (1:500) Takara Cat#632496 Rabbit anti cFos (1:500) Synaptic Systems Cat#226003 Donkey anti Rabbit IgG, Alexa Fluor 488 (1:1000) ThermoFisher Cat#A32790 Donkey anti Rabbit IgG, Rhodamine Red-X (1:1000) Jackson Immunoresearch RRID: AB_2340613 Donkey anti Goat IgG, Alexa Fluor 594 (1:1000) ThermoFisher Cat#A-11058 Donkey anti Chicken IgG, Cy2 (1:1000) Gift from Dr.Shaoyu Ge, Stony Brook University, NY Neurotrace 435/455 (1:500) ThermoFisher Cat#N21479 Bacterial and Virus Strains CAV2-DIO-hM4Di-mCherry Dr.EJ Kremer, Institut de Génétique Moléculaire de Montpellier, France. .. AAV9-PCamk2aGCaMP6f.WPRE.SV40 U. Penn Vector Core AAV8-DIO-hM4Di-mCherry Addgene Cat#44362 AAV9-DIO-eCFP Vector Labs AAV9-GACh4.3 Vigene Biosciences Inc. Stellar Competent Cells, HST08 strain Takara Cat#636766 AAV9-TRE-DIO-oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript AAV9-TRE-DIO-hM4Di-mCherry This Manuscript Chemicals, Peptides, and Recombinant Proteins T4 DNA Ligase NEB Cat#M0202S BglII NEB Cat#R0144S AscI NEB Cat#R0558S BamHI-HF NEB Cat#R3136S PmlI NEB Cat#R0532S Fast Blue Polysciences Inc. Cat#17740-1 doxycycline hyclate-containing chow Envigo Cat#TD.08541 Mt.Lion Pee Maine outoor solutions LLC Clozapine Sigma Aldrich Cat#C6305-25MG Autofluorescence eliminator reagent Millipore Sigma Cat#2160 Phusion® High-Fidelity DNA Polymerase NEB Cat#M0530S Electronic copy available at: https://ssrn.com/abstract=3848894 Critical Commercial Assays In-Fusion® HD Cloning Plus Takara Cat#638920 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Experimental Models: Organisms/Strains Mouse: Chat-IRES-Cre (B6;129S6Chattm2(cre)Lowl/J) The Jackson Laboratory Jax stock number: 006410 Mouse: TetTag (B6;DBA-Tg(FostTA,Fos-EGFP*)1Mmay) The Jackson Laboratory Jax stock number: 008344 Oligonucleotides Chat FWD: TCTGGCAACTTCGTCGGA, REV: CTCCTGGGCTGTTACGCAC IDT pV2.1 - Gene Block 7xTetO-LoxP-Lox2272tTAH100Y.SV40 IDT pV2.2- Gene Block oChIEF-LoxP-Lox2272 IDT Recombinant DNA pAAV-hSyn-DIO-hM4D(Gi)mCherry Addgene, gift from Dr.Bryan Roth Cat#44362 pV2SGE Gift from Dr.Shaoyu Ge, Stony Brook University NY. pAAV-TRE-DIO--oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript pAAV-TRE-DIO-hM4Di-mCherry This Manuscript Software and Algorithms Prism GraphPad Software Inc. Sigmaplot 12.5 Systat Software Inc. OriginPro 9.1 Origin Lab Corporation Fiji is just imagej Fiji FreezeFrame v3 Actimetrics MATLAB Mathworks Pre-processing Analysis MATLAB Script for FiberPhotometry Doric ACh sensor analysis MATLAB script Crouse, Richard B., et al. "Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency." ..

    Blocking Assay:

    Article Title: Basal Forebrain Cholinergic Neurons are Part of the Threat Memory Engram
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti ChAT (1:500) Abcam Cat#ab18736 Rabbit anti GFP (1:1000) Invitrogen Cat#A11122 Chicken anti GFP (1:500) Abcam Cat#ab3970 Mouse anti mCherry (1:500) Takara Cat#632543 Rabbit anti DsRed (1:500) Takara Cat#632496 Rabbit anti cFos (1:500) Synaptic Systems Cat#226003 Donkey anti Rabbit IgG, Alexa Fluor 488 (1:1000) ThermoFisher Cat#A32790 Donkey anti Rabbit IgG, Rhodamine Red-X (1:1000) Jackson Immunoresearch RRID: AB_2340613 Donkey anti Goat IgG, Alexa Fluor 594 (1:1000) ThermoFisher Cat#A-11058 Donkey anti Chicken IgG, Cy2 (1:1000) Gift from Dr.Shaoyu Ge, Stony Brook University, NY Neurotrace 435/455 (1:500) ThermoFisher Cat#N21479 Bacterial and Virus Strains CAV2-DIO-hM4Di-mCherry Dr.EJ Kremer, Institut de Génétique Moléculaire de Montpellier, France. .. AAV9-PCamk2aGCaMP6f.WPRE.SV40 U. Penn Vector Core AAV8-DIO-hM4Di-mCherry Addgene Cat#44362 AAV9-DIO-eCFP Vector Labs AAV9-GACh4.3 Vigene Biosciences Inc. Stellar Competent Cells, HST08 strain Takara Cat#636766 AAV9-TRE-DIO-oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript AAV9-TRE-DIO-hM4Di-mCherry This Manuscript Chemicals, Peptides, and Recombinant Proteins T4 DNA Ligase NEB Cat#M0202S BglII NEB Cat#R0144S AscI NEB Cat#R0558S BamHI-HF NEB Cat#R3136S PmlI NEB Cat#R0532S Fast Blue Polysciences Inc. Cat#17740-1 doxycycline hyclate-containing chow Envigo Cat#TD.08541 Mt.Lion Pee Maine outoor solutions LLC Clozapine Sigma Aldrich Cat#C6305-25MG Autofluorescence eliminator reagent Millipore Sigma Cat#2160 Phusion® High-Fidelity DNA Polymerase NEB Cat#M0530S Electronic copy available at: https://ssrn.com/abstract=3848894 Critical Commercial Assays In-Fusion® HD Cloning Plus Takara Cat#638920 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Experimental Models: Organisms/Strains Mouse: Chat-IRES-Cre (B6;129S6Chattm2(cre)Lowl/J) The Jackson Laboratory Jax stock number: 006410 Mouse: TetTag (B6;DBA-Tg(FostTA,Fos-EGFP*)1Mmay) The Jackson Laboratory Jax stock number: 008344 Oligonucleotides Chat FWD: TCTGGCAACTTCGTCGGA, REV: CTCCTGGGCTGTTACGCAC IDT pV2.1 - Gene Block 7xTetO-LoxP-Lox2272tTAH100Y.SV40 IDT pV2.2- Gene Block oChIEF-LoxP-Lox2272 IDT Recombinant DNA pAAV-hSyn-DIO-hM4D(Gi)mCherry Addgene, gift from Dr.Bryan Roth Cat#44362 pV2SGE Gift from Dr.Shaoyu Ge, Stony Brook University NY. pAAV-TRE-DIO--oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript pAAV-TRE-DIO-hM4Di-mCherry This Manuscript Software and Algorithms Prism GraphPad Software Inc. Sigmaplot 12.5 Systat Software Inc. OriginPro 9.1 Origin Lab Corporation Fiji is just imagej Fiji FreezeFrame v3 Actimetrics MATLAB Mathworks Pre-processing Analysis MATLAB Script for FiberPhotometry Doric ACh sensor analysis MATLAB script Crouse, Richard B., et al. "Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency." ..

    Software:

    Article Title: Basal Forebrain Cholinergic Neurons are Part of the Threat Memory Engram
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti ChAT (1:500) Abcam Cat#ab18736 Rabbit anti GFP (1:1000) Invitrogen Cat#A11122 Chicken anti GFP (1:500) Abcam Cat#ab3970 Mouse anti mCherry (1:500) Takara Cat#632543 Rabbit anti DsRed (1:500) Takara Cat#632496 Rabbit anti cFos (1:500) Synaptic Systems Cat#226003 Donkey anti Rabbit IgG, Alexa Fluor 488 (1:1000) ThermoFisher Cat#A32790 Donkey anti Rabbit IgG, Rhodamine Red-X (1:1000) Jackson Immunoresearch RRID: AB_2340613 Donkey anti Goat IgG, Alexa Fluor 594 (1:1000) ThermoFisher Cat#A-11058 Donkey anti Chicken IgG, Cy2 (1:1000) Gift from Dr.Shaoyu Ge, Stony Brook University, NY Neurotrace 435/455 (1:500) ThermoFisher Cat#N21479 Bacterial and Virus Strains CAV2-DIO-hM4Di-mCherry Dr.EJ Kremer, Institut de Génétique Moléculaire de Montpellier, France. .. AAV9-PCamk2aGCaMP6f.WPRE.SV40 U. Penn Vector Core AAV8-DIO-hM4Di-mCherry Addgene Cat#44362 AAV9-DIO-eCFP Vector Labs AAV9-GACh4.3 Vigene Biosciences Inc. Stellar Competent Cells, HST08 strain Takara Cat#636766 AAV9-TRE-DIO-oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript AAV9-TRE-DIO-hM4Di-mCherry This Manuscript Chemicals, Peptides, and Recombinant Proteins T4 DNA Ligase NEB Cat#M0202S BglII NEB Cat#R0144S AscI NEB Cat#R0558S BamHI-HF NEB Cat#R3136S PmlI NEB Cat#R0532S Fast Blue Polysciences Inc. Cat#17740-1 doxycycline hyclate-containing chow Envigo Cat#TD.08541 Mt.Lion Pee Maine outoor solutions LLC Clozapine Sigma Aldrich Cat#C6305-25MG Autofluorescence eliminator reagent Millipore Sigma Cat#2160 Phusion® High-Fidelity DNA Polymerase NEB Cat#M0530S Electronic copy available at: https://ssrn.com/abstract=3848894 Critical Commercial Assays In-Fusion® HD Cloning Plus Takara Cat#638920 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Experimental Models: Organisms/Strains Mouse: Chat-IRES-Cre (B6;129S6Chattm2(cre)Lowl/J) The Jackson Laboratory Jax stock number: 006410 Mouse: TetTag (B6;DBA-Tg(FostTA,Fos-EGFP*)1Mmay) The Jackson Laboratory Jax stock number: 008344 Oligonucleotides Chat FWD: TCTGGCAACTTCGTCGGA, REV: CTCCTGGGCTGTTACGCAC IDT pV2.1 - Gene Block 7xTetO-LoxP-Lox2272tTAH100Y.SV40 IDT pV2.2- Gene Block oChIEF-LoxP-Lox2272 IDT Recombinant DNA pAAV-hSyn-DIO-hM4D(Gi)mCherry Addgene, gift from Dr.Bryan Roth Cat#44362 pV2SGE Gift from Dr.Shaoyu Ge, Stony Brook University NY. pAAV-TRE-DIO--oChIEF-mCherryP2A-tTAH100Y.SV40 This Manuscript pAAV-TRE-DIO-hM4Di-mCherry This Manuscript Software and Algorithms Prism GraphPad Software Inc. Sigmaplot 12.5 Systat Software Inc. OriginPro 9.1 Origin Lab Corporation Fiji is just imagej Fiji FreezeFrame v3 Actimetrics MATLAB Mathworks Pre-processing Analysis MATLAB Script for FiberPhotometry Doric ACh sensor analysis MATLAB script Crouse, Richard B., et al. "Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency." ..



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